General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences alpha Glucosidase Activity
Glucosidase hydrolyzes carbohydrates by acting on terminal non-reducing a(1A†4)-linked D-glucose residues with the release of D-glucose. Defects in glucosidase have been implicated in Pompe disease and diabetes.
Abcam Cell Migration/Chemotaxis Assay Kit (96-well, 3 ¿m)
Cell Migration/Chemotaxis Assay Kit (96-well, 3 ¿m)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress GLP1R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0300E2CP0M)
The PathHunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TOPOGEN INC ICE Assay Kit (No AB)
TopoGEN has developed and published a method for detecting covalent topo/genomic DNA complexes in live cells that is widely utilized in the field. The method, called the ICE Assay (In vivo Complex of Enzyme) originally required a CsCl gradient to purify genomic DNA and specific topo antibodies to detect trapped topo protein in the DNA fraction. This method, while powerful, is slow, labor intense and requires large expensive equipment. We have modified the technique to allow direct isolation of DNA/topo complexes in a single step, omitting the CsCl gradient step and rapidly increasing the throughput of the method. The ICE Assay can be carried out in a day and a half, and the analysis can be conducted on virtually any cell or tissue system as long as proper controls are performed and mono-specific antibody reagents are used. Our new ICE method is rapid and convenient and can be used with any topoisomerase target (I, II, III).
Abcam Ko-143, BCRP inhibitor, 5MG
MW 469.6 Da. Potent, selective BCRP inhibitor (EC₅₀ values are 23 and 26 nM respectively for the export of mitoxantrone (ab145668, ab141041) and topotecan (ab120388) in cancer cell lines). Non-toxic fumitremorgin C (ab144258) analog. Shows chemopotentiating effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Abcam Total Antioxidant Capacity Assay Kit
Total Antioxidant Capacity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Free Fatty Acid Uptake Assay Kit (Fluorometric)
Free Fatty Acid Uptake Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam SIRT2 Activity Assay Kit (Fluorometric)
SIRT2 Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Hepatic Lipid Accumulation Steatosis Assay Kit
Hepatic Lipid Accumulation Steatosis Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam TMRM Assay Kit (Mitochondrial Membrane Potential)
TMRM Assay Kit (Mitochondrial Membrane Potential)
The product is subject to the following: Abcam Restricted Use Statement